Highlighted findings

Highlighted Findings

JACS Au
2024

Ancestral sequence reconstruction for designing biocatalysts and investigating their functional mechanisms

Biocatalysis has emerged as a green approach for efficient and sustainable production in various industries...

https://doi.org/10.1021/jacsau.4c00653

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RSC Chem Biol
2024 Jun

Enhancement of tryptophan 2-monooxygenase thermostability by semi-rational enzyme engineering: a strategic design to minimize experimental investigation

Tryptophan 2-monooxygenase (TMO) is an FAD-bound flavoenzyme which catalyzes the oxidative decarboxylation of L-tryptophan to produce indole-3-acetamide (IAM) and carbon dioxide...

https://doi.org/10.1039/d4cb00102h

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Angew Chem
2024 Jun

On the Mechanisms of Hypohalous Acid Formation and Electrophilic Halogenation by Non-Native Halogenases

Electrophilic halogenases in nature are typically not efficient. Guided by flavin-dependent halogenase mechanisms and taking advantage of the versatile reactivity of a flavin hydroperoxide adduct and in situ generation of H2O2 by flavin-dependent enzymes...

https://doi.org/10.1002/anie.202403858

J Biol Chem
2024 Feb

A versatile in situ cofactor enhancing system for meeting cellular demands for engineered metabolic pathways

Cofactor imbalance obstructs the productivities of metabolically engineered cells. Herein, we employed a minimally perturbing system...

https://doi.org/10.1016/j.jbc.2023.105598

J Biol Chem
2023 May

High sensitivity and low-cost flavin luciferase (FLUXVc)-based reporter gene for mammalian cell expression

Luciferase-based gene reporters generating bioluminescence signals are important tools for biomedical research...

https://doi.org/10.1016/j.jbc.2023.104639

Nat Catal
2022 Jun

Mechanism-guided tunnel engineering to increase efciency of a favin-dependent halogenase

Although flavin-dependent halogenases (FDHs) are attractive for C–H bond activation, their applications are limited due to low turnover and stability...

https://doi.org/10.1038/s41929-022-00800-8

Angew Chem
2022 Apr

Luciferin Synthesis and Pesticide Detection by Luminescence Enzymatic Cascades

Enzymatic reactions were developed to convert 19 phenolic derivatives containing halogen, nitro, and methyl substituents into eight D-luciferin analogues...

https://doi.org/10.1002/anie.202116908

Chem Rev
2021

Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability

Since the industrial revolution, the rapid growth and development of global industries have depended largely upon the utilization of coal-derived chemicals, and more recently, the utilization of petroleum-based chemicals...

https://doi.org/10.1021/acs.chemrev.1c00121

Angew Chem
2021 Mar

Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments

An efficient and green method is presented for the synthesis of esters using carboxylic acid reductase as a biocatalyst under mild aqueous conditions...

https://doi.org/10.1002/anie.202013962

J Biol Chem
2021 Jan

Dissecting the low catalytic capability of flavin-dependent halogenases

The nicotinamide biomimetic BNAH has been demonstrated to serve as an effective reductant for generating reduced flavin in the chemoenzymatic cascade reaction of bacterial luciferase...

https://doi.org/10.1074/jbc.RA120.016004

Chem Bio Chem
2020 July

A Minimized Chemoenzymatic Cascade for Bacterial Luciferase in Bioreporter Applications

The nicotinamide biomimetic BNAH has been demonstrated to serve as an effective reductant for generating reduced flavin in the chemoenzymatic cascade reaction of bacterial luciferase...

https://doi.org/10.1002/cbic.202000100

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Angew Chem
2019 Sep

A Chemo‐Enzymatic Cascade for the Smart Detection of Nitro‐ and Halogenated Phenols

The flavin‐dependent monooxygenase, HadA, catalyzes the dehalogenation and denitration of the toxicants, nitro‐ and halogenated phenols, to benzoquinone...

https://doi.org/10.1002/anie.201904923

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Chem Sci
2018 Oct

Oxidative dehalogenation and denitration by a flavin-dependent monooxygenase is controlled by substrate deprotonation

Enzymes that are capable of detoxifying halogenated phenols (HPs) and nitrophenols (NPs) are valuable for bioremediation and waste biorefining...

https://doi.org/10.1039/C8SC01482E

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ACS Catalysis
2015 Aug

p-Hydroxyphenylacetate 3-Hydroxylase as a Biocatalyst for the Synthesis of Trihydroxyphenolic Acids

Trihydroxyphenolic acids such as 3,4,5-trihydroxycinnamic acid (3,4,5-THCA) 4c and 2-(3,4,5-trihydroxyphenyl)acetic acid (3,4,5-THPA) 2c are strong antioxidants that are potentially useful as medicinal agents...

https://doi.org/10.1021/acscatal.5b00439

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