Publications

Publications

Journal Articles
JACS
FEBS Journal
  1. Naramittanakul A, Buttranon S, Petchsuk A, Chaiyen P, Weeranoppanant N. Developments of a continuous-flow system with immobilized biocatalysts towards sustainable bioprocessing. React Chem Eng, in press.
  2. Intasian P, Prakinee K, Phintha A, Trisrivirat D, Weeranoppanant N, Wongnate T, Chaiyen P*. Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability. Chem Rev., in press.
  3. Mekseriwattana W, Phungsom A, Sawasdee K, Wongwienkham P, Kuhakarn C, Chaiyen P, Katewongsa KP. Dual Functions of Riboflavin-functionalized Poly(lactic-co-glycolic acid) Nanoparticles for Enhanced Drug Delivery Efficiency and Photodynamic Therapy in Triple-negative Breast Cancer Cells. Photochem Photobiol, in press
  4. Pimviriyakul P, Jaruwat A, Chitnumsub P*, Chaiyen P*. Structural insights into a flavin-dependent dehalogenase HadA explain catalysis and substrate inhibition via quadruple π-stacking. J Biol Chem. 2021 Aug;297(2):100952.
  5. Nearmnala P, Thanaburakorn M, Panbangred W, Chaiyen P, Hongdilokkul N. An in vivo selection system with tightly regulated gene expression enables directed evolution of highly efficient enzymes. Sci Rep. 2021 Jun 3;11(1):11669.
  6. Teanphonkrang S, Suginta W, Sucharitakul J, Fukamizo T, Chaiyen P, Schulte A. An electrochemical method for detecting the biomarker 4-HPA by allosteric activation of Acinetobacterbaumannii reductase C1 subunit. J Biol Chem. 2021 Jan-Jun;296:100467.
  7. Tinikul R, Lawan N, Akeratchatapan N, Pimviriyakul P, Chinantuya W, Suadee C, Sucharitakul J, Chenprakhon P, Ballou DP, Entsch B, Chaiyen P. Protonation status and control mechanism of flavin–oxygen intermediates in the reaction of bacterial luciferase. FEBS J 2021:288(10):3246-3260.
  8. Pongpamorn P, Kiattisewee C, Kittipanukul N, Jaroensuk J, Trisrivirat D, Maenpuen S, and Chaiyen P. Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments. Angew Chem Int Ed Engl 2021 Mar;60(11):5749-53.
  9. Sucharitakul J, Buttranon S, Wongnate T, Chowdhury NP, Prongjit M, Buckel W, Chaiyen P. Modulations of the reduction potentials of flavin-based electron bifurcation complexes and semiquinone stabilities are key to control directional electron flow. FEBS J 2021 Feb;288(3):1008-26.
  10. Kraithong T, Sucharitakul J, Buranachai C, Jeruzalmi D, Chaiyen P, Pakotiprapha D. Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair. DNA Repair (Amst) 2021 Jan;97:103024.
  11. Sucharitakul J, Buckel W, Chaiyen P. Rapid kinetics reveal surprising flavin chemistry in the bifurcating electron transfer flavoprotein from Acidaminococcus fermentans. J Biol Chem. 2021;296:100124.
  12. Phintha A, Prakinee K, Jaruwat A, Lawan N, Visitsatthawong S, Kantiwiriyawanitch C, Songsungthong W, Trisrivirat D, Chenprakhon P, Mulholland AJ, van Pee KH, Chitnumsub P, Chaiyen P. Dissecting the low catalytic capability of flavin-dependent halogenases. J Biol Chem. 2021 Jan;296:100068.
  13. Trisrivirat D, Hughes JMX, Hoeven R, Faulkner M, Toogood H, Chaiyen P, Scrutton NS. Promoter engineering for microbial bio-alkane gas production. Synth Biol (Oxf). 2020 Oct 27;5(1):ysaa022.
  14. Patchsung M, Jantarug K, Pattama A, Aphicho K, Suraritdechachai S, Meesawat P, Sappakhaw K, Leelahakorn N, Ruenkam T, Wongsatit T, Athipanyasilp N, Eiamthong B, Lakkanasirorat B, Phoodokmai T, Niljianskul N, Pakotiprapha D, Chanarat S, Homchan A, Tinikul R, Kamutira P, Phiwkaow K, Soithongcharoen S, Kantiwiriyawanitch C, Pongsupasa V, Trisrivirat D, Jaroensuk J, Wongnate T, Maenpuen S, Chaiyen P, Kamnerdnakta S, Swangsri J, Chuthapisith S, Sirivatanauksorn Y, Chaimayo C, Sutthent R, Kantakamalakul W, Joung J, Ladha A, Jin X, Gootenberg JS, Abudayyeh OO, Zhang F, Horthongkham N, Uttamapinant C. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020;4(12):1140-9.
  15. Chaiyen P, Tamanoi F. Preface: Flavin-Dependent Enzymes: Mechanisms, Structures and Applications. Enzymes. 2020;47:xv-xvi.
  16. Pimviriyakul P, Chaiyen P. Flavin-dependent dehalogenases. Enzymes. 2020;47:365-397.
  17. Phintha A, Prakinee K, Chaiyen P. Structures, mechanisms and applications of flavin-dependent halogenases. Enzymes. 2020;47:327-364.
  18. Chenprakhon P, Pimviriyakul P, Tongsook C, Chaiyen P. Phenolic hydroxylases. Enzymes. 2020; 47:283-326.
  19. Pimviriyakul P, Chaiyen P. Overview of flavin-dependent enzymes. Enzymes. 2020;47:1-36.
  20. Trisrivirat D, Lawan N, Chenprakhon P, Matsui D, Asano Y, Chaiyen P*. Mechanistic insights into the dual activities of the single active site of L-lysine oxidase/monooxygenase from Pseudomonas sp. AIU 813. J Biol Chem. 2020 Aug 7;295(32):11246-11261.
  21. Woraruthai T, Kunno J, Pongsopon M, Yansakon K, Phoopraintra P, Chantiwas R, Leartsakulpanich U, Chaiyen P, Wongnate T. Chaiyen P. Identification and Cultivation of Hydrogenotrophic Methanogens from Palm Oil Mill Effluent for High Methane Production. Int J Energy Res 2020;44:10058-70.
  22. Munkajohnpong P, Kesornpun C, Buttranon S, Jaroensuk J, Weeranoppanant N, Chaiyen P. Fatty Alcohol Production: An Opportunity of Bioprocess. Biofuel Bioprod Bioref 2020;14:986-1009.
  23. Maenpuen S, Pongsupasa V, Pensook W, Anuwan P, Kraivisitkul N, Pinthong C, Phonbuppha J, Luanloet T, Wijma HJ, Fraaije MW, Lawan N, Chaiyen P, Wongnate T. Creating flavin reductase variants with thermostable and solvent-tolerant properties by rational-design engineering. ChemBioChem. 2020;21(10):1481-1491.
  24. Pitsawong W, Chenprakhon P, Dhammaraj T, Medhanavyn D, Sucharitakul J, Tongsook C, van Berkel WJH, Chaiyen P, Miller AF. Tuning of pKa values activates substrates in flavin-dependent aromatic hydroxylases. J Biol Chem. 2020 Mar 20;295(12):3965-3981.
  25. Phonbuppha J, Tinikul R, Wongnate T, Intasian P, Hollmann F, Paul CE, Chaiyen P. A Minimized Chemoenzymatic Cascade for Bacterial Luciferase in Bioreporter Applications. Chembiochem 2020 Jul;21(14):2073-79.
  26. Jaroensuk J, Intasian P, Wattanasuepsin W, Akeratchatapan N, Kesornpun C, Kittipanukul N, Chaiyen P. Enzymatic reactions and pathway engineering for the production of renewable hydrocarbons. J Biotechnol. 2020 Feb 10;309:1-19.
  27. Pimviriyakul P, Wongnate T, Tinikul R, Chaiyen P. Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions. Microb Biotechnol. 2020;13(1):67–86.
  28. Songsungthong W, Yongkiettrakul S, Bohan LE, Nicholson ES, Prasopporn S, Chaiyen P, Leartsakulpanich U. Diaminoquinazoline MMV675968 from Pathogen Box inhibits Acinetobacter baumannii growth through targeting of dihydrofolate reductase. Sci Rep. 2019 Oct;9(1):15625.
  29. Thongdee N, Jaroensuk J, Atichartpongkul S, Chittrakanwong J, Chooyoung K, Srimahaeak T, Chaiyen P, Vattanaviboon P, Mongkolsuk S, Fuangthong M. TrmB, a tRNA m7G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa. Nucleic Acids Res. 2019 Sep;47(17):9271-9281.
  30. Jaroensuk J, Wong YH, Zhong W, Liew CW, Maenpuen S, Sahili AE, Atichartpongkul S, Chionh YH, Nah Q, Thongdee N, McBee ME, Prestwich EG, DeMott MS, Chaiyen P, Mongkolsuk S, Dedon P, Lescar J, Fuangthong M. Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa. RNA. 2019 Nov;25(11):1481-1496.
  31. Watthaisong P, Pongpamorn P, Pimviriyakul P, Maenpuen S, Ohmiya Y, Chaiyen P. A Chemo-Enzymatic Cascade for the Smart Detection of Nitro- and Halogenated Phenols. Angew Chem Int Ed Engl. 2019 Sep;58(38):13254-13258.
    - Highlighted as "Hot Paper:Biocatalysis" and "Frontispiece Article"
    - Highlighted in more than 8 news outlets
  32. Pongpamorn P, Watthaisong P, Pimviriyakul P, Jaruwat A, Lawan N, Chitnumsub P, Chaiyen P. Identification of a Hotspot Residue for Improving the Thermostability of a Flavin-Dependent Monooxygenase. Chembiochem. 2019 Dec 13;20(24):3020-3031.
  33. Jaroensuk J, Intasian P, Kiattisewee C, Munkajohnpon P, Chunthaboon P, Buttranon S, Trisrivirat D, Wongnate T, Maenpuen S, Tinikul R, Chaiyen P. Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway. J Biol Chem. 2019 Jul 26;294(30):11536-11548.
  34. Ubonprasert S, Jaroensuk J, Pornthanakasem W, Kamonsutthipaijit N, Wongpituk P, Mee-Udorn P, Rungrotmongkol T, Ketchart O, Chitnumsub P, Leartsakulpanich U, Chaiyen P, Maenpuen S. A flap motif in human serine hydroxymethyl transferase is important for structural stabilization, ligand binding, and control of product release. J Biol Chem. 2019 Jul 5;294(27):10490-10502.
  35. Duangpummet P, Chaiyen P, Chenprakhon P. Lipase-Catalyzed Esterification: An Inquiry-Based Laboratory Activity to Promote High School Students' Understanding and Positive Perceptions of Green Chemistry. J Chem Educ. 2019 May;96(6):1205-1211.
  36. Teanphonkrang S, Ernst A, Janke S, Chaiyen P, Sucharitakul J, Suginta W, Khunkaewla P, Schuhmann W, Schulte A, Ruff A. Amperometric detection of the urinary disease biomarker p-HPA by allosteric modulation of a redox polymer-embedded bacterial reductase. ACS Sens. 2019 May 24;4(5):1270-1278.
  37. Wongnate T, Surawatanawong P, Chuaboon L, Lawan N, Chaiyen P. The mechanism of sugar C-H bond oxidation by a flavoprotein oxidase occurs by a hydride transfer before proton abstraction. Chemistry. 2019 Mar 21;25(17):4460-4471.
  38. Chuaboon L, Wongnate T, Punthong P, Kiattisewee C, Lawan N, Hsu CY, Lin CH, Bornscheuer U, Chaiyen P. One-Pot Bioconversion of L-Arabinose to L-Ribulose in an Enzymatic Cascade. Angew Chem Int Ed Engl. 2019 Feb 18;58(8):2428-2432.
  39. Chenprakhon P, Wongnate T, Chaiyen P. Monooxygenation of aromatic compounds by flavin-dependent monooxygenases. Protein Sci. 2019 Jan;28(1):8-29.
  40. Pimviriyakul P, Chaiyen P. A complete bioconversion cascade for dehalogenation and denitration by bacterial flavin-dependent enzymes. J Biol Chem. 2018 Nov 30;293(48):18525-18539.
  41. Pimviriyakul P, Surawatanawong P, Chaiyen P. Oxidative dehalogenation and denitration by a flavin-dependent monooxygenase is controlled by substrate deprotonation. Chem Sci. 2018;9:7468-82.
  42. Yuenyaw A, Petchyam N, Kamonsutthipaijit N, Chaiyen P and Pakotiprapha D. Crystal structure of the flavin reductase of Acinetobacter baumannii p-hydroxyphenylacetate 3-hydroxylase (HPAH) and identification of amino acid residues underlying its regulation by aromatic ligands. Arch Biochem Biophys. 2018 Sep 1; 653:24-38.
  43. Phonbuppha J, Maenpuen S, Munkajohnpong P, Chaiyen P, Tinikul R. A selective determination of the catalytic cysteine pKa of 2-cysteine succinic semialdehyde dehydrogenase from Acinetobacter baumannii using burst kinetics and enzyme adduct formation. FEBS J. 2018 Jul;285(13):2504-2519.
  44. Schwertz G, Witschel MC, Rottmann M, Leartsakulpanich U, Chitnumsub P, Jaruwat A, Amornwatcharapong W, Ittarat W, Schäfer A, Aponte RA, Chaiyen P, and Diederich F. Potent Inhibitors for Plasmodial Serine Hydroxymethyltransferase (SHMT) Featuring a Spirocyclic Scaffold. ChemMedChem. 2018 May 8;13(9):931-943.
  45. Teanphonkrang S, Janke S, Chaiyen P, Sucharitakul J, Suginta W, Khunkaewla P, Schuhmann W, Ruff A, Schulte A. Tuned Amperometric Detection of Reduced β-Nicotinamide Adenine Dinucleotide by Allosteric Modulation of the Reductase Component of the p-Hydroxyphenylacetate Hydroxylase Immobilized within a Redox Polymer. Anal Chem. 2018 May;90(9):5703–5711.
  46. Şahin S, Wongnate T, Chuaboon L, Chaiyen P, Yu EH. Enzymatic fuel cells with an oxygen resistant variant of pyranose-2-oxidase as anode biocatalyst. Biosens Bioelectron. 2018 Jun;107:17-25.
  47. Tinikul R, Chenprakhon P, Maenpuen S, Chaiyen P. Biotransformation of Plant-Derived Phenolic Acids. Phenolic Acids. Biotechnol J. 2018 Jun;13(6):e1700632.
  48. Sawasdee K, Sucharitakul J, Dhammaraj T, Niamsiri N, Chaiyen P, Prapainop K Encapsulation of the reductase component of p-Hydroxyphenylacetate Hydroxylase in poly (lactide-co-glycolide) nanoparticles by three different emulsification techniques. IET Nanobiotechnology. 2018 Jun;12(4):423-428.
  49. Schwertz G, Frei MS, Witschel MC, Rottmann M, Leartsakulpanich U, Chitnumsub P, Jaruwat A, Ittarat A, Schäfer A, Aponte RA, Trapp N, Mark K, Chaiyen P, Diederich F. Conformational Aspects in the Design of Inhibitors for Serine Hydroxymethyltransferase (SHMT): Biphenyl, Aryl Sulfonamide, and Aryl Sulfone Motifs. Chemistry. 2017 Oct 12;23(57):14345-14357.
  50. Pinthong C , Phoopraintra P, Chantiwas R, Pongtharangkul T, Chenprakhon P, and Chaiyen P. Green and Sustainable Biocatalytic Production of 3,4,5-Trihydroxycinnamic Acid from Palm Oil Mill Effluent. Process Biochem. 2017;63:122-129.
  51. Amornwatcharapong W, Maenpuen S, Chitnumsub P, Leartsakulpanich P, and Chaiyen P. Human and Plasmodium Serine Hydroxymethyl Transferases Differ in Rate-limiting Steps and pH-Dependent Substrate Inhibition Behavior. Arch Biochem Biophys 2017;630:91-100.
  52. Schwertz G, Witschel MC, Rottmann M, Bonnert R, Leartsakulpanich U, Chitnumsub P, Jaruwat A, Ittarat W, Schäfer A, Aponte RA, Charman SA, White KL, Kundu A, Sadhukhan S, Lloyd M, Freiberg GM, Srikumaran M, Siggel M, Zwyssig A, Chaiyen P, Diederich F. Antimalarial Inhibitors Targeting Serine Hydroxymethyltransferase (SHMT) with in Vivo Efficacy and Analysis of their Binding Mode Based on X-ray Cocrystal Structures. J Med Chem. 2017 Jun 22;60(12):4840-4860.
  53. Chenprakhon P, Dhammaraj T, Chantiwas R, Chaiyen P. Hydroxylation of 4-hydroxyphenylethylamine derivatives by R263 variants of the oxygenase component of p-hydroxyphenylacetate-3-hydroxylase. Arch Biochem Biophys. 2017 Apr 15;620:1-11.
  54. Pimviriyakul P, Thotsaporn K, Sucharitakul J and Chaiyen P. Kinetic Mechanism of the Dechlorinating Flavin-Dependent Monooxygenase HadA. J Biol Chem. 2017 Mar 24;292(12):4818-4832.
  55. Pornsuwan S, Maenpuen S, Kamutira P, Watthaisong P, Thotsaporn K, Tongsook C, Juttulapa M, Nijvipakul S, Chaiyen P. 3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe(II)-containing enzyme with fast turnover. PLOS ONE 2017 Feb;12:e0171135.
  56. Pruksatrakul T, PPhoopraintra P, Wilairat P, Chaiyen P, Chantiwas R. Development of a sequential injection-liquid microextraction procedure with GC-FID for analysis of short-chain fatty acids in palm oil mill effluent. Talanta 2017;165(1):612-618.
  57. Thotsaporn K, Tinikul R, Maenpuen S, Phonbuppha J, Watthaisong P, Chenprakhon P, and Chaiyen P. Enzymes in the p-hydroxyphenylacetate degradation pathway of Acinetobacter baumannii. J Mol Catal B Enzym 2018;134:353–366.
  58. Dhammaraj T, Pinthong C, Visitsatthawong S, Tongsook C, Surawatanawong P, Chaiyen P. A Single-site Mutation at Ser146 Expands the Reactivity of the Oxygenase Component of p-Hydroxyphenylacetate 3-Hydroxylase. ACS Chem Biol. 2016;11(10):2889-2896.
  59. Tinikul R and Chaiyen P. Structure, Mechanism, and Mutation of Bacterial Luciferase. Adv Biochem Eng Biotechnol. 2016;154:47-74.
  60. Sucharitakul J, Medhanavyn D, Pakotiprapha D, van Berkel W. Chaiyen P. Tyr217 and His213 are Important for Substrate Binding and Hydroxylation of 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii. FEBS J 2016 Mar;283(5):860-81.
  61. Graf M, Sucharitakul J, Bren U, Chu B, Koellensberger G; Hann S, Furtmüller P, Obinger C, Peterbauer C, Oostenbrink C. Chaiyen P, Haltrich, D. Reaction of pyranose dehydrogenase from Agaricus meleagris with its carbohydrate substrates. FEBS J. 2015 Nov;282(21):4218-41.
  62. Chaiyen P, Scrutton NS. Special Issue: Flavins and Flavoproteins: Introduction. FEBS J. 2015 Aug;282(16):3001-2.
  63. Visitsatthawong S, Chenprakhon P, Chaiyen P, and Surawatanawong P. Mechanism of oxygen activation in a flavin-dependent monooxygenase: A nearly barrierless C4a-hydroperoxyflavin formation via Proton-coupled electron transfer. J Am Chem Soc 2015;137(29):9363-74.
  64. Dhammaraj T, Phintha A, Pinthong C, Medhanavyn D, Tinikul R, Chenprakhon P, Sucharitakul J, Vardhanabhuti N, Jiarpinitnun C, and Chaiyen P. p-Hydroxyphenylacetate 3-hydroxylase as a biocatalyst for the synthesis of trihydroxyphenolic acids. ACS Catalysis 2015; 5:4492–4502.
  65. Witschel MC, Rottmann M, Schwab A, Leartsakulpanich U, Chitnumsub P, Seet M, Tonazzi S, Schwertz G, Stelzer F, Mietzner T, McNamara C, Thater F, Freymond C, Jaruwat A, Pinthong C, Riangrungroj P, Oufir M, Hamburger M, Mäser P, Sanz-Alonso LM, Charman S, Wittlin S, Yuthavong Y, Chaiyen P, Diederich F. Inhibitors of plasmodial serine hydroxymethyltransferase (SHMT): Cocrystal structures of pyrazolopyrans with potent blood- and liver-stage activities. J Med Chem 2015;58(7):3117-30.
  66. Maenpuen S, Watthaisong P, Supon P, Sucharitakul J, Parsonage D, Karplus PA, Claiborne A, Chaiyen P. Kinetic Mechanism of L-alpha-Glycerophosphate Oxidase from Mycoplasma pneumoniae. FEBS J. 2015; 282(16):3043-59.
  67. Maenpuen S, Amornwatcharapong W, Krasatong P, Sucharitakul J, Palfey BA, Yuthavong Y, Chitnumsub P, Leartsakulpanich U, Chaiyen P. Kinetic mechanism and the rate-limiting step of Plasmodium vivax serine hydroxymethyltransferase. J Biol Chem 2015;290(13):8656-65.
  68. Elkhal C.K., Kean K, Parsonage D, Maenpuen S, Chaiyen P, Claiborne A and Karplus P.A. Structure and proposed mechanism of alpha-glycerophosphate oxidase from Mycoplasma pneumonia. FEBS J. 2015; 282(16):3030-42.
  69. Luanloet T, Sucharitakul J and Chaiyen P*. Selectivity of Substrate Binding and Ionization of 2-Methyl-3-Hydroxypyridine-5-Carboxylic Acid Oxygenase. FEBS J. 2015; 282(16):3107-25.
  70. Panatdasirisuk, W., Vongsetskul, T., Sucharitakul, J., Chaiyen, P., Tangboriboonrat, P. Functionalized electrospun regenerated cellulose fibers for immobilizing pyranose 2-oxidase. React Funct Polym 2015;86:47-51.
  71. Messiha HL, Wongnate T, Chaiyen P, Jones AR, Scrutton NS. Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes. J R Soc Interface. 2015;12(103).
  72. Sahin S, Wongnate T, Chaiyen P, Yu EH. Glucose oxidation using oxygen resistant pyranose-2-oxidase for biofuel cell applications. Chem Eng Trans 2014;41:367-372.
  73. Chitnumsub P, Jaruwat A, Riangrungroj P, Ittarat W, Noytanom K, Oonanant W, Vanichthanankul J, Chuankhayan P, Maenpuen S, Chen CJ, Chaiyen P, Yuthavong Y, Leartsakulpanich U. Structures of Plasmodium vivax serine hydroxymethyltransferase: Implications for ligand-binding specificity and functional control. Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3177-86.
  74. Chenprakhon P , Trisrivirat D, Thotsaporn K , Sucharitakul J, Chaiyen P*. Control of C4a-hydroperoxyflavin protonation in the oxygenase component of p-hydroxyphenyl acetate-3-hydroxylase. Biochemistry 2014 Jul 1;53(25):4084-6. (Rapid Report)
  75. Srisook T, Vongsetskula T*, Sucharitakul J, Chaiyen P, Tangboriboonrat P. Immobilization of 3-hydroxybenzoate 6-hydroxylase onto functionalized electrospun polycaprolactone ultrafine fibers: a novel heterogeneous catalyst. React Funct Polym 2014 Sep;82:41-46.
  76. Sucharitakul J, Tinikul R, Chaiyen P. Mechanisms of Reduced Flavin Transfer in the Two-component flavin-dependent Monooxygenases. Arch Biochem Biophys. 2014 Aug;555-556:33-46.
  77. Pinthong C, Maenpuen S, Amornwatcharapong W, Yuthavong Y, Leartsakulpanich U, Chaiyen P.Distinct Biochemical Properties of Human Serine Hydroxymethyltransferase Compared to the Plasmodium Enzyme: Implications for Selective Inhibition. FEBS J. 2014 Jun;281(11):2570-83.
  78. Chitnumsub P, Ittarat P, Jaruwat A, Noytanom K, Amornwatcharapong W, Pornthanakasem W, Chaiyen P, Yuthavong Y, Leartsakulpanich U. Structure of Plasmodium falciparum serine hydroxymethyltransferase reveals a novel redox switch that regulates its activities. Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1517-27.
  79. Wongnate T, Surawatanawong P, Visitsatthawong S, Sucharitakul J, Scrutton NS, Chaiyen P. Proton-coupled Electron Transfer and Adduct Configuration are Important for C4a-Hydroperoxyflavin Formation and Stabilization in a Flavoenzyme. J Am Chem Soc. 2014;136(1):241–253.
  80. Sucharitakul J*, Tongsook C, Pakotiprapha D, van Berkel WJ, Chaiyen P. The Reaction Kinetics of 3-Hydroxybenzoate 6-Hydroxylase from Rhodoccocus jostii RHA1 Provide an Understanding of the para-Hydroxylation Enzyme Catalytic Cycle. J Biol Chem. 2013 Dec 6;288:35210-35221.
  81. Tinikul R, Pitsawong W, Sucharitakul J, Nijvipakul S, Ballou DP, Chaiyen P*. The transfer of reduced FMN from LuxG oxidoreductase to luciferase occurs via free diffusion. Biochemistry 2013 Oct 1;52(39):6834-6843.
  82. Wongnate T, Chaiyen P*. The substrate oxidation mechanism of pyranose 2-oxidase and other related enzymes in the glucose-methanol-choline superfamily. FEBS J 2013 Jul;280(13):3009-27. (Review)
  83. Prongjit M, Sucharitakul J, Palfey BA, Chaiyen P*. Oxidation mode of pyranose 2-oxidase is controlled by pH. Biochemistry 2013 Feb 26;52(8):1437-45.
  84. Tan TC, Spadiut O, Wongnate T, Sucharitakul J, Krondorfer I, Sygmund C, Haltrich D, Chaiyen P, Peterbauer CK, Divne C*. The 1.6 Å crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate Specificity and Reveals a Flavin Intermediate. PLoS One 2013;8(1):e53567.
  85. Tinikul R, Thotsaporn K, Taveekarn V, Jitrapakdee S, Chaiyen P. The fusion Vibrio campbellii luciferase as a eukaryotic gene reporter. J Biotechnol 162 (2012):pp 346–353.
  86. Chaiyen P, Fraaije M, Mattevi A. The Enigmatic Reaction of Flavins with Oxygen. Trends Biochem Sci 2012;37(9):373-80.
  87. Taniguchi S, Chosrowjan H, Wongnate T, Sucharitakul J, Chaiyen P, Tanaka F. Ultrafast Fluorescence Dynamics of Flavin Adenine Dinucleotide in Pyranose 2-Oxidases Variants and Their Complexes with Acetate: Conformational Heterogeneity with Different Dielectric Constants. J Photochem Photobiol A Chem 2012;245:33-42.
  88. Phongsak T, Sucharitakul J, Thotsaporn K, Oonanant W, Yuvaniyama J, Svasti J, Ballou DP, Chaiyen P.The C-terminal domain of 4-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii is an auto-inhibitory domain. J Biol Chem 2012; 287(31):26213-26222.
  89. Sopitthummakhun K, Thongpanchang C, Vilaivan T, Yuthavong Y, Chaiyen P, Leartsakulpanich U. Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay. Malaria J 2012;11(1):194.
  90. Somprasong N, Jittawuttipoka T, Duang-nkern J, Romsang, A, Chaiyen P, Schweizer H, Vattanaviboon P, Mongkolsuk S. Pseudomonas aeruginosa thiol peroxidase protects against hydrogen peroxide toxicity and displays atypical patterns of gene regulation. J Bacteriol 2012;194(15):3904-12.
  91. Sucharitakul J, Wongnate T, Montersino S, van Berkel, WJH, Chaiyen P. Reduction Kinetics of 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii RHA1. Biochemistry 2012; 51(21):4309-21.
  92. Oonnanat W, Sucharitakul J, Chaiyen P, Yuaniyama J. Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii. Acinetobacter baumannii. Acta Crystallogr Sect F Struct Biol Cryst Commun 2012 Jun;68:720–723.
  93. Chosrowjan H, Taniguchi S, Wongnate T, Sucharitakul J, Chaiyen P, Tanaka F. Conformational heterogeneity in pyranose 2-oxidase from Trametes multicolor revealed by ultrafast fluorescence dynamics. J Photochem Photobiol A Chem 2012;234(April 15):44-48.
  94. Chenprakhon P, Panijpan B, Chaiyen P. An Experiment Illustrating the Change in Ligand pKa upon Protein Binding. J Chem Edu 2012; 89:791−795.
  95. Tongsook C, Sucharitakul J, Thotsaporn K, Chaiyen P. Interactions with the substrate phenolic group are essential for hydroxylation by the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase. J Biol Chem 2011;286(52):44491–502.
  96. Wongnate T, Sucharitakul J , and Chaiyen P. Identification of a catalytic base for sugar oxidation in pyranose 2-oxidase reaction. Chembiochem 2011;12(17):2577-86.
  97. Penpassakarn P, Chaiyen P, Palittapongarnpim P. Efficient heterologous expression and one-step purification of fully active c-terminal histidine-tagged uridine monophosphate kinase from Mycobacterium tuberculosis. Southeast Asian J Trop Med Public Health 2011;42(6):1452-1459.
  98. Thotsaporn K, Chenprakhon P, Sucharitakul J, Mattevi A, Chaiyen P. Stabilization of C4A-hydroperoxy-flavin in a two-component flavin-dependent monooxygenase is achieved through interactions at flavin N5 and C4a atoms. J Biol Chem 2011;286(32):28170-80.
  99. Sucharitakul J, Wongnate T, Chaiyen P. Hydrogen peroxide elimination from C4A-hydroperoxy-flavin in a flavoprotein oxidase occurs through a single proton transfer from flavin N5 to a peroxide leaving group. J Biol Chem 2011;286(19):16900-9.
  100. Ruangchan N, Tongsook C, Sucharitakul J, Chaiyen P. pH-dependent studies reveal an efficient hydroxylation mechanism of the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase. J Biol Chem 2011;286(1):223-33.
  101. Nijvipakul S, Ballou SP, Chaiyen P. Reduction Kinetics of a Flavin Oxidoreductase LuxG from Photobacterium leiognathi (TH1):Half Sites Reactivity. Biochemistry 2010;49 (43):9241–8.
  102. Tan TC, Pitsawong W, WongnateT, Spadiut O, Haltrich D, Chaiyen P, Divne C. H-bonding and Positive Charge at the N(5)/O(4) Locus are Critical for Covalent Flavin Attachment in Trametes Pyranose 2-Oxidase. J Mol Biol 2010;402(3):578-94.
  103. Rujkorakarn R, Nunthaboot N, Tanaka F, Chaiyen P, Chosrowjan H, Taniguchi S, Mataga N. Time-resolved Stokes shift in proteins with continuum model: Slow dynamics in proteins. J Photochem Photobiol A Chem 2010;215(1):38-45.
  104. Chenprakhon P, Sucharitakul J, Panijpan B, Chaiyen P. Measuring binding affinity of protein. Ligand interaction using spectrophotometry: binding of neutral red to riboflavin-binding protein. J Chem Edu 2010;87(8):829-31.
  105. Sucharitakul J, Wongnate T, Chaiyen P. Kinetic isotope effects on the noncovalent flavin mutant protein of pyranose 2-oxidase reveal insights into the flavin reduction mechanism. Biochemistry 2010;49(17):3753-65.
  106. Pitsawong W, Sucharitakul J, Prongjit M, Tan TC, Spadiut O, Haltrich D, Divne C, Chaiyen P. A conserved active-site threonine is important for both sugar and flavin oxidations of pyranose 2-oxidase. J Biol Chem 2010;285(13):9697-705.
  107. Chaiyen P. Flavoenzymes catalyzing oxidative aromatic ring-cleavage reactions. Arch Biochem Biophys. 2010;493(1):62-70. (Review)
  108. Maenpuen S, Sopitthammakhun K, Yuthavong Y, Chaiyen P, Leartsakulpanich U. Characterization of Plasmodium falciparum serine hydroxymethyltransferase—A potential antimalarial target. Mol Biochem Parasitol 2009;168(1):63-73.
  109. Sopitthammakhun K, Maenpuen S, Yuthavong Y, Leartsakulpanich U, Chaiyen P. Serine hydroxymethyltransferase from Plasmodium vivax is different in substrate specificity from its homologues. FEBS J 2009;276(15):4023-36.
  110. Baron R, Riley C, Chenprakhon P, Thotsaporn K, Winter RT, Alfieri A, Forneris F, van Berkel WJH, Chaiyen P, Fraaije MW, Mattevi A, McCammon JA. Multiple pathways guide oxygen diffusion into flavoenzyme active sites. Proc Natl Acad Sci U S A 2009;106(25):10603-8.
    [ Cited in "Research Highlights" Nature Chemistry, 2009]
  111. Chosrowjan H, Taniguchi S, Mataga N, Phongsak T, Sucharitakul J, Chaiyen P, Tanaka F. Ultrafast Solvation Dynamics of flavin mononucleotide in the reductase component of p-Hydroxyphenylacetate Hydroxylase. J Phys Chem B 2009;113(25):8439-42.
  112. Prongjit M, Sucharitakul J, Wongnate T, Haltrich D, Chaiyen P. Kinetic mechanism of pyranose 2-oxidase from Trametes multicolor. Biochemistry 2009;48(19):4170-80.
  113. Spadiut O, Pisanelli I, Maischberger T, Peterbauer C, Gorton L, Chaiyen P, Haltrich D. Engineering of pyyranose 2-oxidase: improvement for biofuel cell and food applications through semi-rational protein design. J Biotechnol 2009; 139(3):250-7.
  114. Sucharitakul J, Prongjit M, Haltrich D, Chaiyen P. Detection of a C4a-Hydroperoxyflavin Intermediate in the Reaction of a Flavoprotein Oxidase. Biochemistry 2008;47(33):8485–90.
  115. Nijvipakul S, Wongratana J, Suadee C, Entsch B, Ballou DP, Chaiyen P. LuxG is a functioning flavin reductase for bacterial luminescence. J Bacteriol 2008;190(5):1531-8.
  116. Sucharitakul J, Phongsak T, Entsch B, Svasti J, Chaiyen P, Ballou DP. Kinetics of a two-component p-hydroxyphenylacetate hydroxylase explain how reduced flavin is transferred from the reductase to the oxygenase. Biochemistry 2007;46(29):8611-23.
  117. Suadee C, Nijvipakul S, Svasti J, Entsch B, Ballou DP, Chaiyen P. Luciferase from Vibrio campbellii is more thermostable and binds reduced FMN better than its homologues. J Biochem 2007;142(4):539-52.
  118. Alfieri A, Fersini F, Ruangchan N, Prongjit M, Chaiyen P, Mattevi A. Structure of the monooxygenase component of a two-component flavoprotein monooxygenase. Proce Natl Acad Scie U S A 2007;104(4):1177-82.
  119. Sucharitakul J, Chaiyen P, Entsch B, Ballou DP. Kinetic mechanisms of the oxygenase from a two-component enzyme, p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii. J Biol Chem 2006;281(25):17044-53.
  120. Kujawa M, Ebner H, Leitner C, Hallberg BM, Prongjit M, Sucharitakul J, Ludwig R, Rudsander U, Peterbauer C, Chaiyen P, Haltrich D, Divne C. Structural basis for substrate binding and regioselective oxidation of monosaccharides at C3 by pyranose 2-oxidase. J Biol Chem 2006;281(46):35104-15.
  121. Sucharitakul J, Chaiyen P, Entsch B, Ballou DP. The reductase of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii requires p-hydroxyphenylacetate for effective catalysis. Biochemistry 2005;44(30):10434-42.
  122. Oonanant W, Sucharitakul J, Yuvaniyama J, Chaiyen P. Crystallization and preliminary X-ray crystallographic analysis of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase from Pseudomonas sp. MA-1. Acta Crystallogr Sect F Struct Biol Cryst Commun 2005;61(3):312-4.
  123. Hommalai G, Chaiyen P, Svasti J. Studies on the transglucosylation reactions of cassava and Thai rosewood β-glucosidases using 2-deoxy-2-fluoro-glycosyl-enzyme intermediates. Arch Biochem Biophys 2005;442(1):11-20.
  124. Thotsaporn K, Sucharitakul J, Wongratana J, Suadee C, Chaiyen P. Cloning and expression of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii: Evidence of the divergence of enzymes in the class of two-protein component aromatic hydroxylases. Biochim Biophys Acta 2004;1680(1):60-6.
  125. Chaiyen P, Sucharitakul J, Svasti J, Entsch B, Massey V, Ballou DP. Use of 8-Substituted-FAD Analogues to Investigate the Hydroxylation Mechanism of the Flavoprotein 2-Methyl-3-hydroxypyridine-5-carboxylic Acid Oxygenase. Biochemistry 2004;43(13):3933-43.
  126. Chaiyen P, Suadee C, Wilairat P. A novel two-protein component flavoprotein hydroxylase p-hydroxyphenylacetate hydroxylase from Acinetobacter baumannii. Eur J Biochem 2001;268(21):5550-61.
  127. Chaiyen P, Brissette P, Ballou DP, Massey V. Thermodynamics and reduction kinetics properties of 2-methyl-3- hydroxypyridine-5-carboxylic acid oxygenase. Biochemistry 1997;36(9):2612-21.
  128. Chaiyen P, Brissette P, Ballou DP, Massey V. Unusual mechanism of oxygen atom transfer and product rearrangement in the catalytic reaction of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase. Biochemistry 1997;36(26):8060-70.
  129. Chaiyen P, Ballou DP, Massey V. Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase. Proc Nat Acad Sci U S A 1997;94(14):7233-8.
  130. Chaiyen P, Brissette P, Ballou DP, Massey V. Reaction of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase with N-methyl-5-hydroxynicotinic acid: Studies on the mode of binding, and protonation status of the substrate. Biochemistry 1997;36(45):13856-64.

Chapter in Book

Editor of the Book: The Enzymes, Volume 47. Flavin-Dependent Enzymes (published on 1st October 2020)
8 chapters in books published by Walter de Gruyter GmbH, Springer-Verlag Berlin-Heidelberg, Wiley-VCH Verlag GmbH & Co, Academic Press and Elsevier.

  • Maenpuen S, Tinikul S, Chenprakhon P and Chaiyen P. Production of Valuable Phenolic Compounds from Lignin by Biocatalysis: State-of-the-Art Perspective. In Emerging Areas in Bioengineering (2018), Wiley-VCH Verlag GmbH & Co. pp 102-124.
  • Tinikul R and Chaiyen P. Structure, Mechanism, and Mutation of Bacterial Luciferase In: Advances in Biochemical Engineering/Technology (Springer-Verlag Berlin Heidelberg) 2016; 154:47-74.
  • Wongnate T and Chaiyen, P. Pyranose oxidases. In: Hille R., Miller, S.M., Palfey, B. (Eds.). Handbook of Flavoproteins. Oxidases, dehydrogenases and related systems. Volume 1. 2013. p. 177-193.
  • Chaiyen P, Suadee C, Meevoothisom V. Degradation of phenylacetic acid by bacteria from Thai soil. In: Annual Report of ICBiotech. Vol. 21. Osaka: International Center for Biotechnology; 1998. p. 862-7.